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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

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Standard procedures were followed to generate transgenic mice by microinjection of the purified expression cassette into the pronuclei of one-cell-stage embryos and their subsequent re-transfer into the oviducts of pseudopregnant recipient females.
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In these experiments CTSL-knockout keratinocytes were more responsive to EGF than wild-type keratinocytes.
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In the present study, we chose murine EGF as the model ligand to investigate the EGF-receptor system of CTSL-deficient keratinocytes.
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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

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Discussion Regulated proliferation is a key aspect of keratinocyte biology.
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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

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Two major hypotheses, each with very significant implications for regulation of epidermal cell homeostasis, can be drawn from these experiments.
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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

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